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KMID : 0613820180280060648
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Journal of Life Science
2018 Volume.28 No. 6 p.648 ~ p.655
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Enhancement of Testosterone in TM3 Leydig Cells by an Eritadenine-containing Agaricus blazei Mycelial Liquid Culture Extract
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Kim Young-Suk
Jung Jae-Eun
Jeong Hui-Jeong
Moon Yeon-Kyu
Kim Jeong-Ok
Ha Yeong-Lae
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Abstract
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Enhancement mechanistic actions of testosterone (TS) productions in mouse Leydig TM3 cells by the eritadenine (EA) and/or the Agaricus blazei mycelial liquid culture extract (ABMLCE). Productions of TS in TM3 cells were investigated in normal and oxidative-stressed culture conditions. In the normal culture condition, TM3 cells grown in a Dulbecco’s Modified Eagle’s Medium (DMEM) were treated with EA (0~100 ppm) and ABMLCE (10 ppm) + EA (0~50 ppm) for 24 hr, and in the oxidative-stressed culture condition, the cells grown in DMEM containing 50 μM H2O2 to induce oxidative stress for 4 h were treated with the same as those in the normal culture condition. TS content, 3β-hydroxysteroid dehydrogenase 2 (HSD3B2) enzyme activity, 5α-reductase 2 (5α-R2) enzyme activity, and free-radical nitric oxide (NO) content in the culture media were measured using their corresponding assay kits. EA, ABMLCE, and ABMLCE + EA significantly, p<0.05, enhanced TS productions in both cultural conditions, relative to control treatment. The activity of the HSD3B2 enzyme, which is involved in the production of precursors for TS production, was elevated by EA, ABMLCE, and ABMLCE + EA treatments in both culture conditions. The activity of the 5α-R2 enzyme, which converts TS to dihydroxytestosterone (DHT), was not significantly affected in either culture condition by EA, ABMLCE, or ABMLCE + EA treatments. The treatments included reduced NO content. These results indicate that EA, ABMLCE, and EA + ABMLCE treatments elevated TS in TM3 cells via the enhancements of HSD3B2 activity and the reduction of NO production, and also imply that EA and ABMLCE or EA + ABMLCE could be useful materials for the production of TS in humans.
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KEYWORD
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Leydig TM3 cell, nitric oxide, testosterone, 3β-hydroxysteroid dehydrogenase 2, 5α-reductase
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